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This product employs a magnetic microparticle chemiluminescence method, utilizing a double-antibody sandwich assay to detect samples through a two-step immunoassay.
The first step involves adding the sample, HCV-cAg magnetic microparticles, and sample diluent to a reaction tube for incubation. The HCV antigen in the sample binds to the coated HCV Core antibody. After the reaction, magnetic separation and washing are performed to remove unbound portions.
The second step involves adding horseradish peroxidase-labeled HCV Core antibody to form an antibody-antigen-antibody-enzyme complex. After the reaction, magnetic separation and washing are performed again to remove unbound portions.
The third step involves adding the chemiluminescent substrate solution to the reaction tube. Luminol reacts with hydrogen peroxide under the catalysis of horseradish peroxidase to produce a chemiluminescent reaction. The chemiluminescence intensity (RLU) is detected using a photomultiplier tube, and the intensity is positively correlated with the content of the hepatitis C virus core antigen.
